HELA PRODUCTS
Cell line
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.
Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.
All our Hela products :
Cell Pellets
Cell pellets are prepared by low speed centrifugation, rinsed with phosphate-buffered saline, snap frozen in liquid nitrogen and stored at -85°C. The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.
Nuclei
Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Nuclei are then snap frozen in liquid nitrogen and stored at -85°C.
Nuclear Extracts
The Nuclear Extracts are prepared according to:
Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489
Nuclear Extract (not dialysed)
No dialysis is performed during the preparation of this product. The preparation stopped after the ultra-centrifugation step. Buffer composition is then the same as the extraction buffer
Cytoplasmic Extract
Cytoplasmic Extracts is a rich source of complexes (40S, 60S ribosomal subunits and 80S ribosomes etc…), Factors involved in translation (eIFs’, IRES- and PolyA binding proteins), Factors which play a role on different parts of the mRNA molecule (5′- and 3′ UTRs’).
Our HeLa S100 Cytoplasmic Extracts are suitable for use in in vitro splicing; protein-DNA/RNA and protein-protein interactions; source of individual splicing factors and other regulatory proteins.
Mitochondria
Our Hela Mitochondria are suitable for use in human mitochondrial processes studies; purification of mitochondrial DNA;purification and isolation of mitochondrial proteins (ERK1/2, mitochondrial ribosomes, …).