Nuclei are prepared from cells rinsed with phosphate buffer saline by low speed centrifugation. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Nuclei are then snap frozen in liquid nitrogen and stored at -85°C.
All our products of nuclei :
CV – 1
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.