HeLa Nuclear Extracts
€90,00 – €920,00
Volume:from 150ul to 15ml. Concentration:~6mg/ml (Bradford). Hela Nuclear Extracts are prepared from fresh cells in exponential phase only (Viability>95%).
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.
Cultures are screened for the presence of bacteria, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.
HeLa Nuclear Extracts Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.
The Nuclear Extracts are prepared according to:
Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489
Conc. Protein (Bradford) = +/- 6 mg/ml
Buffer used for dialysis of our Nuclear Extracts is:
- HEPES 20mM
- KCl 100mM
- EDTA 0.2mM
- Glycerol 20%
- PMSF 0.2mM
- DTT 0.5mM
Our HeLa Nuclear Extracts are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.
Source of HDAC activity, transcription factors, chromatin proteins and histones.
Also suitable for use in:
- separation by SDS-PAGE;
- Gel Shift assays used for protein-DNA interactions studies;
- HDAC assays;
- positive control for Western Blot assays;
- in vitro splicing;
- transcription factors studies;
- cell division cycle and apoptosis studies;
- spliceosome and other proteome studies (DNA ends, snRNP’s, DNA PKcs, …).
Downloads – Documents
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- Stefan Reber, Jolanda Stettler1, Giuseppe Filosa, Martino Colombo Daniel Jutzi, Silvia C Lenzken, Christoph Schweingruber, Rémy Bruggmann, Angela Bachi, Silvia ML Barabino, Oliver Mühlemann & Marc-David Ruepp (2016) Minor intron splicing is regulated by FUS and affected by ALS-associated FUS mutants. The EMBO Journal 35: 1504–1521
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- Katarzyna Dorota Raczynska Marc-David Ruepp Aleksandra Brzek Stefan Reber Valentina Romeo Barbara Rindlisbacher Manfred Heller Zofia Szweykowska-Kulinska Artur Jarmolowski Daniel Schümperli. (2015) FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors. Nucleic Acids Research, Volume 43, Issue 20, 16 November 2015, Pages 9711–9728
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- Takashi Ochi, Andrew N. Blackford, Julia Coates, Satpal Jhujh, Shahid Mehmood, Naoka Tamura, Jon Travers, Qian Wu, Viji M. Draviam, Carol V. Robinson, Tom L. Blundell, and Stephen P. Jackson (2015) PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015 Jan 9; 347(6218): 185–188.
- Andrew N. Blackford, Jadwiga Nieminuszczy, Rebekka A. Schwab, Yaron Galanty, Stephen P. Jackson, and Wojciech Niedzwiedz (2015) TopBP1 Interacts with BLM to Maintain Genome Stability but Is Dispensable for Preventing BLM Degradation. Mol Cell. 2015 Mar 19; 57(6): 1133–1141
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- Cell Pellets (12)
- Cytoplasm (2)
- Hela Products (6)
- Mitochondria (1)
- Nuclear Extract (1)
- Nuclear Extract (Not dialysed) (1)
- Nuclei (2)
- S100 (1)