Cell pellets are prepared by low speed centrifugation of cell culture supensions, they are rinsed with phosphate-buffered saline, snap frozen in liquid nitrogen and stored at -85°C. The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.
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HUT – 78
Derived from the peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2 and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for HUT-78.
Human plasma cell leukemia established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described to be EBNA-negative and to express mRNA for proto-oncogene bcl-2 confirmed as human with IEF of AST, LDH, PEP B.
U-937 cells are a model cell line used in biomedical research. They were isolated from the histiocytic lymphoma of a 37 year old male patient and are used to study the behaviour and differentiation of monocytes. U937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morphology and characteristics of mature macrophages.