HELA PRODUCTS

Cell Line

The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.

Production

HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

All our Hela products

Cell Pellets

Cell pellets are prepared by low speed centrifugation of cell culture supensions, they are rinsed with phosphate-buffered saline, snap frozen in liquid nitrogen and stored at -85°C. The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

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Nuclei

Nuclei are prepared from cells rinsed with phosphate buffer saline by low speed centrifugation. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Nuclei are then snap frozen in liquid nitrogen and stored at -85°C.

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Nuclear Extracts

The Nuclear Extracts are prepared according to slightly adapted protocol from: Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489

Final Protein Conc. (Bradford) = +/- 6 mg/ml.

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Nuclear Extracts

(not dialysed)

The Nuclear Extracts are prepared according to slightly adapted protocol from: Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-148

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Cytoplasmic Extract

Cytoplasmic Extracts is a rich source of complexes (40S, 60S ribosomal subunits and 80S ribosomes etc…), Factors involved in translation (eIFs’, IRES- and PolyA binding proteins), Factors which play a role on different parts of the mRNA molecule (5′- and 3′ UTRs’).

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S-100 Cytoplasmic Extract

Our HeLa S100 Cytoplasmic Extracts are suitable for use in in vitro splicing; protein-DNA/RNA and protein-protein interactions; source of individual splicing factors and other regulatory proteins.

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Mitochondria

Mitochondria extract are isolated from nuclei and other organelle by differential centrifugations. After adding a suspension buffer made up of sucrose, mitochondria are then snap frozen in liquid nitrogen and stored at -85°C. Our extract are suitable for in vitro studies of mitochondrial respiration, apoptosis, cellular signaling as well as DNA or mitochondrial protein purification.

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