Hela Nuclear Extracts
€300,00 – €575,00
Hela cells pellets harvest in exponential phase and Nuclear Extracts are prepared from fresh cells only.
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.
HeLa Nuclear Extracts Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.
The Nuclear Extracts are prepared according to :
Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489
Conc. Protein (Bradford) = +/- 6 mg/ml
Buffer used for dialysis of our Nuclear Extracst is:
- HEPES 20mM
- KCl 100mM
- EDTA 0.2mM
- Glycerol 20%
- PMSF 0.2mM
- DTT 0.5mM
Our HeLa Nuclear Extracts are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. Also suitable for use in Gel Shift and Western Blotting assay. For published papers reporting the use of our extract, please check out our references list
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.