The Nuclear Extracts are prepared according to slightly adapted protocol from: Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Nucleic Acids Res. 11, 1475-1489
Final Protein Conc. (Bradford) = +/- 6 mg/ml.
Our products of Nuclear Extracts Not Dialysed :
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Belgium. Cells are harvested in exponential phase.