Quality Control
Cultures are screened for the presence of bacteria, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.

HeLa S100 Cytoplasmic Extracts Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Ipracell, provider of high quality HeLa Nuclear Extract (HNE) expands its activities by making available the cytoplasm prepared from exponentially growing HeLa cells (HeLa Cytoplasmic Extract: HCE).
HCE is a rich source of:

• complexes (40S, 60S ribosomal subunits and 80S ribosomes etc…)
• Factors involved in translation (eIFs’, IRES- and PolyA binding proteins).
• Factors which play a role on different parts of the mRNA molecule (5′- and 3′ UTRs’).

It can be used to study the regulation of muRNA processing, mRNA polymorphism etc…
Preliminary data suggest that HCE compares favorably with the rabbit reticulocyte lysate for cell-free protein expression.
HCE production automation makes it possible to offer this subcellular fraction of high and reproducible quality at very competitive prices.

Research Use
Our HeLa S100 Cytoplasmic Extracts are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

Also suitable for use in:

• in vitro splicing;
• protein-DNA/RNA and protein-protein interactions;
• source of individual splicing factors and other regulatory proteins.

Downloads – Documents

References

• Plessel, G., Fischer, U. and Lührmann, R, (1994). m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (SnRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)­methyltransferase is a non-SnRNP cytoplasmic protein that requires a binding site on the Sm core domain. Molecular and Cellular Biology 14, 4160-4172.