Nuclei – Ipracell https://www.ipracell.be Production & exploitation of mammalian cells Thu, 08 Jan 2026 12:09:28 +0000 en-US hourly 1 https://wordpress.org/?v=6.7.4 https://www.ipracell.be/wp-content/uploads/2025/02/cropped-Favicon-32x32.png Nuclei – Ipracell https://www.ipracell.be 32 32 CV-1 Nuclei https://www.ipracell.be/product/cv-1-nuclei/ Fri, 02 Mar 2018 11:35:37 +0000 http://www.ipracell.be/?post_type=product&p=353 CV-1 cell line
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
Derivation
The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies.

CV-1 Nuclei Production
CV-1 cells are grown in cell-factories under GLP conditions in our facility in Mons, Belgium.

Nuclei are prepared by low speed centrifugation, rinsed with phosphate buffer saline. After exposure to hypotonic buffer, nuclei are separated from cytoplasm and membrane using dounce and centrifugation. Nuclei are then snap frozen in liquid nitrogen and stored at -85°C.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).

]]> HeLa Nuclei https://www.ipracell.be/product/hela-nuclei/ Thu, 13 Apr 2017 12:24:14 +0000 http://new.ipracell.be/?post_type=product&p=160 Hela cells pellets harvest in exponential phase and Nuclei are prepared from fresh cells only.

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The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). NBCS used in the culture medium is certified from New Zealand origin.

HeLa Nuclei Production
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×106 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Research Use
Our HeLa Nuclei are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

Also suitable for use in:

  • entities production and purification of biological material from human origin used in many other applications (as core histones used in HAT assays);
  • Gel Shift and Western Blotting assay;
  • biochemical processing;
  • nucleic acid purification and research;
  • protein expression studies;
  • in vitro activity assays.

References

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